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dc.contributor.authorEduardo, Iban
dc.contributor.authorAlegre, Simó
dc.contributor.authorAlexiou, Konstantinos G.
dc.contributor.authorArús, Pere
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2020-11-02T15:49:23Z
dc.date.available2020-11-02T15:49:23Z
dc.date.issued2020-08-07
dc.identifier.citationEduardo, Iban, Simó Alegre, Konstantinos G. Alexiou, and Pere Arús. 2020. "Resynthesis: Marker-Based Partial Reconstruction Of Elite Genotypes In Clonally-Reproducing Plant Species". doi:10.1101/2020.05.09.085738.ca
dc.identifier.issn1664-462Xca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/953
dc.description.abstractWe propose a method for marker-based selection of cultivars of clonally-reproducing plant species which keeps the basic genetic architecture of a top-performing cultivar (usually a partly heterozygous genotype), with the addition of some agronomically relevant differences (such as production time, product appearance or quality), providing added value to the product or cultivation process. The method is based on selecting a) two complementary nearly-inbred lines from successive selfing generations (ideally only F2 and F3) of large size, that may generate individuals with most of their genome identical to the original cultivar but being homozygous for either of the two component haplotypes in the rest, and b) individuals with such characteristics already occurring in the F2. Option a) allows for introgressing genes from other individuals in one or both of these nearly-inbred lines. Peach, a woody-perennial, clonally-reproduced species, was chosen as a model for a proof of concept of the Resynthesis process due to its biological characteristics: self-compatibility, compact and genetically well-known genome, low recombination rates and relatively short intergeneration time (3–4 years). From 416 F2 seedlings from cultivar Sweet Dream (SD), we obtained seven individuals with 76–94% identity with SD, and selected five pairs of complementary lines with average homozygosity of the two parents ≥0.70 such that crossing would produce some individuals highly similar to SD. The application of this scheme to other species with more complex genomes or biological features, including its generalization to F1 hybrids, is discussed.ca
dc.format.extent8ca
dc.language.isoengca
dc.publisherFrontiers Mediaca
dc.relation.ispartofFrontiers in Plant Scienceca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleResynthesis: Marker-Based Partial Reconstruction of Elite Genotypes in Clonally-Reproducing Plant Speciesca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO-FEDER/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-40228-C02-01/ES/USO DE LA SECUENCIA GENOMICA PARA LA CARACTERIZACION DE LA VARIABILIDAD INTRACLONAL E INTERESPECIFICA EN MELOCOTONERO Y ALMENDRO/ca
dc.relation.projectIDINIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00050-00-00/ES/Nuevas estrategias de mejora del melocotonero basadas en marcadores moleculares: introgresión asistida por marcadores y resíntesis/ca
dc.relation.projectIDMINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /ca
dc.subject.udc633ca
dc.identifier.doihttps://doi.org/10.3389/fpls.2020.01205ca
dc.contributor.groupFructiculturaca
dc.contributor.groupGenòmica i Biotecnologiaca


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