dc.contributor.author | Toldrà, Anna | |
dc.contributor.author | O'Sullivan, Ciara K. | |
dc.contributor.author | Diogène, Jorge | |
dc.contributor.author | Campàs, Mònica | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2020-11-02T16:39:03Z | |
dc.date.available | 2022-08-10T22:45:11Z | |
dc.date.issued | 2020-08-10 | |
dc.identifier.citation | Toldrà, Anna, Ciara K. O'Sullivan, Jorge Diogène, and Mònica Campàs. 2020. "Detecting Harmful Algal Blooms With Nucleic Acid Amplification-Based Biotechnological Tools". Science Of The Total Environment 749: 141605. doi:10.1016/j.scitotenv.2020.141605. | ca |
dc.identifier.issn | 0048-9697 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/957 | |
dc.description.abstract | Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nucleic acid-based (molecular) methods are being considered for the unequivocal identification of microalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nucleic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process (from sample procurement to in-situ implementation) are described. | ca |
dc.format.extent | 27 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Science of The Total Environment | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/Dispositivos bioanalíticos para detección rápida y rentable de gambierdiscus y ciguatoxinas/CIGUASENSISNG | ca |
dc.subject.udc | 574 | ca |
dc.identifier.doi | https://doi.org/10.1016/j.scitotenv.2020.141605 | ca |
dc.contributor.group | Aigües Marines i Continentals | ca |