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dc.contributor.authorToldrà, Anna
dc.contributor.authorAndree, Karl B.
dc.contributor.authorBertomeu, Edgar
dc.contributor.authorRoque, Ana
dc.contributor.authorCarrasco, Noèlia
dc.contributor.authorGairín, Ignasi
dc.contributor.authorFurones, M. Dolores
dc.contributor.authorCampàs, Mònica
dc.contributor.otherProducció Animalca
dc.date.accessioned2018-12-03T14:05:05Z
dc.date.available2018-12-03T14:05:05Z
dc.date.issued2018-10-03
dc.identifier.citationToldrà, A., Andree, K., Bertomeu, E., Roque, A., Carrasco, N., & Gairín, I. et al. (2018). Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads. PLOS ONE, 13(10), e0205207. doi:10.1371/journal.pone.0205207ca
dc.identifier.issn1932-6203ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/98
dc.description.abstractOstreid herpesvirus-1 (OsHV-1) has been involved in mass mortality episodes of Pacific oysters Crassostrea gigas throughout the world, causing important economic losses to the aquaculture industry. In the present study, magnetic beads (MBs) coated with an anionic polymer were used to capture viable OsHV-1 from two types of naturally infected matrix: oyster homogenate and seawater. Adsorption of the virus on the MBs and characterisation of the MB-virus conjugates was demonstrated by real-time quantitative PCR (qPCR). To study the infective capacity of the captured virus, MB-virus conjugates were injected in the adductor muscle of naïve spat oysters, using oyster homogenate and seawater without MBs as positive controls, and bare MBs and sterile water as negative controls. Mortalities were induced after injection with MB-virus conjugates and in positive controls, whereas no mortalities were recorded in negative controls. Subsequent OsHV-1 DNA and RNA analysis of the oysters by qPCR and reverse transcription qPCR (RT-qPCR), respectively, confirmed that the virus was the responsible for the mortality event and the ability of the MBs to capture viable viral particles. The capture of viable OsHV-1 using MBs is a rapid and easy isolation method and a promising tool, combined with qPCR, to be applied to OsHV-1 detection in aquaculture facilities.ca
dc.format.extent15ca
dc.language.isoengca
dc.publisherPublic Library of Scienceca
dc.relation.ispartofPLoS ONEca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleRapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beadsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDEC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDIca
dc.subject.udc639 - Caça. Pesca. Pisciculturaca
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0205207ca
dc.contributor.groupAigües Marines i Continentalsca
dc.contributor.groupAqüiculturaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/