Skin Multi-Omics-Based Interactome Analysis: Integrating the Tissue and Mucus Exuded Layer for a Comprehensive Understanding of the Teleost Mucosa Functionality as Model of Study
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Author
Reyes-López, Felipe E.
Ibarz, Antoni
Ordóñez-Grande, Borja
Vallejos-Vidal, Eva
Andree, Karl B.
Balasch, Joan Carles
Fernández-Alacid, Laura
Sánchez-Nuño, Sergio
Firmino, Joana P.
Pavez, Leonardo
Polo, Javier
Tort, Lluis
Publication date
2021-02-04ISSN
1664-3224
Abstract
From a general structural perspective, a mucosal tissue is constituted by two main matrices: the tissue and the secreted mucus. Jointly, they fulfill a wide range of functions including the protection of the epithelial layer. In this study, we simultaneously analyzed the epithelial tissue and the secreted mucus response using a holistic interactome-based multi-omics approach. The effect of the gilthead sea bream (Sparus aurata) skin mucosa to a dietary inclusion of spray-dried porcine plasma (SDPP) was evaluated. The epithelial skin microarrays-based transcriptome data showed 194 differentially expressed genes, meanwhile the exuded mucus proteome analysis 35 differentially synthesized proteins. Separately, the skin transcripteractome revealed an expression profile that favored biological mechanisms associated to gene expression, biogenesis, vesicle function, protein transport and localization to the membrane. Mucus proteome showed an enhanced protective role with putatively higher antioxidant and antimicrobial properties. The integrated skin mucosa multi-interactome analysis evidenced the interrelationship and synergy between the metabolism and the exuded mucus functions improving specifically the tissue development, innate defenses, and environment recognition. Histologically, the skin increased in thickness and in number of mucous cells. A positive impact on animal performance, growth and feed efficiency was also registered. Collectively, the results suggest an intimate crosstalk between skin tissue and its exuded mucus in response to the nutritional stimulus (SDPP supplementation) that favors the stimulation of cell protein turnover and the activation of the exudation machinery in the skin mucosa. Thus, the multi-omics-based interactome analysis provides a comprehensive understanding of the biological context of response that takes place in a mucosal tissue. In perspective, this strategy is applicable for evaluating the effect of any experimental variable on any mucosal tissue functionality, including the benefits this assessment may provide on the study of the mammalian mucosa.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
637 - Produce of domestic (farmyard) animals and game
Pages
20
Publisher
Frontiers Media
Is part of
Frontiers in Immunology
Citation
Reyes-López, Felipe E., Antoni Ibarz, Borja Ordóñez-Grande, Eva Vallejos-Vidal, Karl B. Andree, Joan Carles Balasch, and Laura Fernández-Alacid et al. 2021. "Skin Multi-Omics-Based Interactome Analysis: Integrating The Tissue And Mucus Exuded Layer For A Comprehensive Understanding Of The Teleost Mucosa Functionality As Model Of Study". Frontiers In Immunology 11. doi:10.3389/fimmu.2020.613824.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-51839-C5-5-R/ES/Fuentes proteicas y tratamientos alternativos para mejorar la sostenibilidad de los cultivos de dorada y lenguado/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-70637-R/ES/Caracterización funcional del mucus epidermico en peces de interés productivo: metodologia no invasiva de identificación de biomarcadores y relación con el estadio fisiológico/
MAPAMA/JACUMAR/ /ES/Estrategias nutricionales para la mejora en el rendimiento productivo: uso de piensos funcionales y dietas salud en acuicultura/DIETAplus
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/117RT0521/ES/Estrategias de desarrollo y mejora de la producción de larvas de peces en Iberoamerica/LARVAplus
EC/H2020/727315/EU/Mediterranean Aquaculture Integrated Development/MedAID
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2820]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/