High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo
Visualitza/Obre
Autor/a
Auray, Gaël
Talker, Stephanie C.
Keller, Irene
Python, Sylvie
Gerber, Markus
Liniger, Matthias
Bruggmann, Rémy
Ruggli, Nicolas
Summerfield, Artur
Data de publicació
2020-07-07ISSN
1664-3224
Resum
The present study investigated the transcriptomic response of porcine dendritic cells (DC) to innate stimulation in vitro and in vivo. The aim was to identify DC subset-specialization, suitable Toll-like receptor (TLR) ligands targeting plasmacytoid DC (pDC), and the DC activation profile during highly and low virulent classical swine fever virus (CSFV, strain Eystrup and Pinar del Rio, respectively) infection, chosen as model for a virus causing a severe immunopathology. After identification of porcine conventional DC (cDC) 1, cDC2, pDC and a monocyte-derived subset in lymphoid tissues, we characterized DC activation using transcriptomics, and focused on chemokines, interferons, cytokines, as well as on co-stimulatory and inhibitory molecules. We demonstrate that porcine pDC provide important signals for Th1 and interferon responses, with CpG triggering the strongest responses in pDC. DC isolated early after infection of pigs with either of the two CSFV strains showed prominent upregulation of CCL5, CXCL9, CXCL10, CXCL11, and XCL1, as well as of the cytokines TNFSF13B, IL6, IL7, IL12B, IL15, IL27. Transcription of IL12B and many interferon genes were mostly restricted to pDC. Interestingly, the infection was associated with a prominent induction of inhibitory and cell death receptors. When comparing low and highly virulent CSFV strains, the latter induced a stronger inflammatory and antiviral response but a weaker cell cycle response, and reduced antigen presentation functions of DC. Taken together, we provide high-resolution information on DC activation in pigs, as well as information on how DC modulation could be linked to CSFV immunopathology.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
22
Publicat per
Frontiers Media
Publicat a
Frontiers in Immunology
Citació
Auray, Gaël, Stephanie C. Talker, Irene Keller, Sylvie Python, Markus Gerber, Matthias Liniger, Llilianne Ganges, Rémy Bruggmann, Nicolas Ruggli and Artur Summerfield. 2020. “High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo”. Front. Immunol. 11: 1429. doi: 10.3389/fimmu.2020.01429.
Programa
Sanitat Animal
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