Esterase, Glutathione S-Transferase and NADPH-Cytochrome P450 Reductase Activity Evaluation in Cacopsylla pyri L. (Hemiptera: Psyllidae) Individual Adults
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Fecha de publicación
2021-04-07ISSN
2075-4450
Resumen
Cacopsylla pyri (L.) (Hemiptera: Psyllidae) is a key pest of pear orchards in Spain. The large number of insecticide treatments necessary for control may be an important contributor to the emergence of resistance. Laboratory toxicity and biochemical assays are necessary to validate the existence of insecticide resistance and establish the underlying mechanisms. All the methodologies developed to evaluate enzyme activity in C. pyri to date have incorporated “pools” of adults to detect minimum activity ranges. In this study, we determined the optimal working conditions for evaluation of the activities of esterase, glutathione S-transferase and NADPH-cytochrome P450 reductase in individual insects via colorimetric methods using a microplate reader. The main factors affecting enzymatic analysis activity, such as enzyme source and substrate concentration, filter wavelength, buffer pH, reaction time and additives, were evaluated for optimization. Determining the frequency of resistant individuals within a population could be used as an indicator for the evolution of insecticide resistance over time. Two laboratory strains, one of them selected with cypermethrin, and two field populations were analyzed for this purpose. The data obtained revealed high values and great variation in the activity ranges of esterase (EST) in the insecticide-selected population as well as in the field populations validating the applied methodology
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
632 - Enfermedades y protección de las plantas
Páginas
17
Publicado por
MDPI
Publicado en
Insects
Citación
Bosch-Serra, Dolors, Marcela A. Rodríguez, Jesús Avilla, María José Sarasúa, and Xavier Miarnau. 2021. "Esterase, Glutathione S-Transferase And NADPH-Cytochrome P450 Reductase Activity Evaluation In Cacopsylla Pyri L. (Hemiptera: Psyllidae) Individual Adults". Insects 12 (4): 329. doi:10.3390/insects12040329.
Número del acuerdo de la subvención
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-77373-C2-1-R/ES/Avances en el control intregrado de plagas en cultivos hortofrutícolas: mejora del control biológico por conservación en hortícolas y frutales/CIPTOOLS
Program
Protecció Vegetal Sostenible
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