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dc.contributor.authorVilanova, Laura
dc.contributor.authorValero-Jiménez, Claudio A.
dc.contributor.authorvan Kan, Jan A.L.
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2021-05-25T11:08:10Z
dc.date.available2021-05-25T11:08:10Z
dc.date.issued2021-04-14
dc.identifier.citationVilanova, Laura, Claudio A. Valero-Jiménez, and Jan A.L. van Kan. 2021. "Deciphering The Monilinia Fructicola Genome To Discover Effector Genes Possibly Involved In Virulence". Genes 12 (4): 568. doi:10.3390/genes12040568.ca
dc.identifier.issn2073-4425ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1274
dc.description.abstractBrown rot is the most economically important fungal disease of stone fruits and is primarily caused by Monilinia laxa and Monlinia fructicola. Both species co-occur in European orchards although M. fructicola is considered to cause the most severe yield losses in stone fruit. This study aimed to generate a high-quality genome of M. fructicola and to exploit it to identify genes that may contribute to pathogen virulence. PacBio sequencing technology was used to assemble the genome of M. fructicola. Manual structural curation of gene models, supported by RNA-Seq, and functional annotation of the proteome yielded 10,086 trustworthy gene models. The genome was examined for the presence of genes that encode secreted proteins and more specifically effector proteins. A set of 134 putative effectors was defined. Several effector genes were cloned into Agrobacterium tumefaciens for transient expression in Nicotiana benthamiana plants, and some of them triggered necrotic lesions. Studying effectors and their biological properties will help to better understand the interaction between M. fructicola and its stone fruit host plants.ca
dc.format.extent15ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofGenesca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDeciphering the Monilinia fructicola Genome to Discover Effector Genes Possibly Involved in Virulenceca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDEC/H2020/741964/EU/The role of necrotrophic effectors in the ability of Botrytis and Monilinia species to infect host plants/NECROFUNGIca
dc.subject.udc633ca
dc.identifier.doihttps://doi.org/10.3390/genes12040568ca
dc.contributor.groupPostcollitaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/