Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties
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Author
Jin, Jing
Gan, Kexin
Zhao, Lan
Jia, Huijuan
Zhu, Yifan
Li, Xiongwei
Yang, Zhaowei
Ye, Zhengwen
Cao, Ke
Wang, Zhiqiang
Yu, Mingliang
Zhang, Yuyan
Ma, Zhisheng
Liu, Hangkong
Akkerdaas, Jaap H.
Gao, Zongshan
van Ree, Ronald
Publication date
2021-05-14ISSN
2045-7022
Abstract
Background: Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties.
Methods: To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme‐linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction.
Results: A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20–73.44 μg/g, fresh weight), being very low in the pulp (0.05–9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late‐ripening yellow flesh varieties were usually highly allergenic.
Conclusion: Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, “Zi Xue Tao,” “Wu Yue Xian,” and “May Fire,” were identified, which could be useful in trials for peach allergy patients.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
10
Publisher
Wiley Open Access
Is part of
Clinical and Translational Allergy
Citation
Jin, Jing, Kexin Gan, Lan Zhao, Huijuan Jia, Yifan Zhu, Xiongwei Li, and Zhaowei Yang et al. 2021. "Peach Allergen Pru P 1 Content Is Generally Low In Fruit But With Large Variation In Different Varieties". Clinical And Translational Allergy 11 (3). doi:10.1002/clt2.12034.
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2811]
Rights
Copyright © 2021 The Authors. Clinical and Translational Allergy published by John Wiley and Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/