M448R and MGF505-7R: Two African Swine Fever Virus Antigens Commonly Recognized by ASFV-Specific T-Cells and with Protective Potential
Visualitza/Obre
Autor/a
Bosch-Camós, Laia
López, Elisabet
Collado, Javier
Navas, María J.
Salas, Maria Luisa
Mundt, Egbert
Nikolin, Veljko
Rodríguez, Fernando
Data de publicació
2021-05-14ISSN
2076-393X
Resum
African swine fever (ASF) is today′s number one threat for the global swine industry. Neither commercial vaccine nor treatment is available against ASF and, thus far, only live attenuated viruses (LAV) have provided robust protection against lethal ASF virus (ASFV) challenge infections. Identification of ASFV proteins inducing protective immune responses is one of the major challenges to develop safer and efficient subunit vaccines. Immunopeptidomic studies recently performed in our laboratory allowed identifying ASFV antigens recognized by ASFV-specific CD8+ T-cells. Here, we used data from the SLAI-peptide repertoire presented by a single set of ASFV-infected porcine alveolar macrophages to generate a complex DNA vaccine composed by 15 plasmids encoding the individual peptide-bearing ORFs. DNA vaccine priming improved the protection afforded by a suboptimal dose of the BA71ΔCD2 LAV given as booster vaccination, against Georgia2007/1 lethal challenge. Interestingly, M448R was the only protein promiscuously recognized by the induced ASFV-specific T-cells. Furthermore, priming pigs with DNA plasmids encoding M488R and MGF505-7R, a CD8+ T-cell antigen previously described, confirmed these two proteins as T-cell antigens with protective potential. These studies might be useful to pave the road for designing safe and more efficient vaccine formulations in the future.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
16
Publicat per
MDPI
Publicat a
Vaccines
Citació
Bosch-Camós, Laia, Elisabet López, Javier Collado, María J. Navas, Miguel Blanco-Fuertes, Sonia Pina-Pedrero, and Francesc Accensi et al. 2021. "M448R And MGF505-7R: Two African Swine Fever Virus Antigens Commonly Recognized By ASFV-Specific T-Cells And With Protective Potential". Vaccines 9 (5): 508. https://www.mdpi.com/2076-393X/9/5/508/htm.
Número de l'acord de la subvenció
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-78160-C2-1-R/ES/Estrategias de protección frente a la peste porcina africana: de la investigación básica a la aplicada/
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-107616RB-I00/ES/Peste porcina africana; de la emergencia a evitar el endemismo/
Programa
Sanitat Animal
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