Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
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Author
Ancín, María
Larraya, Luis
Bénard, Camille
Fernández-San Millán, Alicia
Veramendi, Jon
Gibon, Yves
Fernie, Alisdair R.
Aranjuelo, Iker
Farran, Inmaculada
Publication date
2021-05-08ISSN
0022-0957
Abstract
In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
16
Publisher
Oxford University Press
Is part of
Journal of Experimental Botany
Citation
Ancín, María, Luis Larraya, Igor Florez-Sarasa, Camille Bénard, Alicia Fernández-San Millán, Jon Veramendi, Yves Gibon, Alisdair R Fernie, Iker Aranjuelo, and Inmaculada Farran. 2021. "Overexpression Of Thioredoxin M In Chloroplasts Alters Carbon And Nitrogen Partitioning In Tobacco". Journal Of Experimental Botany 72 (13): 4949-4964. doi:10.1093/jxb/erab193.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-79868-R/ES/Nuevos criterios de mejora aplicados al incremento en la producción y de calidad de trigo duro en un contesto de cambio climático/
EC/H2020/753301/EU/Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening/ARCATOM
MICIU-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2829]
Rights
Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/