dc.contributor.author | Ancín, María | |
dc.contributor.author | Larraya, Luis | |
dc.contributor.author | Florez-Sarasa, Igor | |
dc.contributor.author | Bénard, Camille | |
dc.contributor.author | Fernández-San Millán, Alicia | |
dc.contributor.author | Veramendi, Jon | |
dc.contributor.author | Gibon, Yves | |
dc.contributor.author | Fernie, Alisdair R. | |
dc.contributor.author | Aranjuelo, Iker | |
dc.contributor.author | Farran, Inmaculada | |
dc.contributor.other | Producció Vegetal | ca |
dc.date.accessioned | 2021-12-09T11:12:48Z | |
dc.date.available | 2021-12-09T11:12:48Z | |
dc.date.issued | 2021-05-08 | |
dc.identifier.citation | Ancín, María, Luis Larraya, Igor Florez-Sarasa, Camille Bénard, Alicia Fernández-San Millán, Jon Veramendi, Yves Gibon, Alisdair R Fernie, Iker Aranjuelo, and Inmaculada Farran. 2021. "Overexpression Of Thioredoxin M In Chloroplasts Alters Carbon And Nitrogen Partitioning In Tobacco". Journal Of Experimental Botany 72 (13): 4949-4964. doi:10.1093/jxb/erab193. | ca |
dc.identifier.issn | 0022-0957 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/1443 | |
dc.description.abstract | In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts. | ca |
dc.format.extent | 16 | ca |
dc.language.iso | eng | ca |
dc.publisher | Oxford University Press | ca |
dc.relation.ispartof | Journal of Experimental Botany | ca |
dc.rights | Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-79868-R/ES/Nuevos criterios de mejora aplicados al incremento en la producción y de calidad de trigo duro en un contesto de cambio climático/ | ca |
dc.relation.projectID | EC/H2020/753301/EU/Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening/ARCATOM | ca |
dc.relation.projectID | MICIU-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/ | ca |
dc.subject.udc | 633 | ca |
dc.identifier.doi | https://doi.org/10.1093/jxb/erab193 | ca |
dc.contributor.group | Genòmica i Biotecnologia | ca |