Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
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Author
Te, Nigeer
Creve, Rhea
Pérez, Mónica
Vergara-Alert, Júlia
Publication date
2022-09-02ISSN
0928-4249
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
7
Publisher
BMC
Is part of
Veterinary Research
Citation
Te, Nigeer, Jordi Rodon, Rhea Creve, Mónica Pérez, Joaquim Segalés, Júlia Vergara-Alert, and Albert Bensaid. 2022. "Evaluation Of Alpaca Tracheal Explants As An Ex Vivo Model For The Study Of Middle East Respiratory Syndrome Coronavirus (MERS-Cov) Infection". Veterinary Research 53 (1). doi:10.1186/s13567-022-01084-3.
Grant agreement number
EC/H2020/731014/EU/Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation /VetBioNet
EC/PF7/115760/EU/Zoonotic Anticipation and Preparedness Initiative/ZAPI
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2685]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/