Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
Ver/Abrir
Autor/a
Te, Nigeer
Creve, Rhea
Pérez, Mónica
Vergara-Alert, Júlia
Fecha de publicación
2022-09-02ISSN
0928-4249
Resumen
Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
619 - Veterinaria
Páginas
7
Publicado por
BMC
Publicado en
Veterinary Research
Citación
Te, Nigeer, Jordi Rodon, Rhea Creve, Mónica Pérez, Joaquim Segalés, Júlia Vergara-Alert, and Albert Bensaid. 2022. "Evaluation Of Alpaca Tracheal Explants As An Ex Vivo Model For The Study Of Middle East Respiratory Syndrome Coronavirus (MERS-Cov) Infection". Veterinary Research 53 (1). doi:10.1186/s13567-022-01084-3.
Número del acuerdo de la subvención
EC/H2020/731014/EU/Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation /VetBioNet
EC/PF7/115760/EU/Zoonotic Anticipation and Preparedness Initiative/ZAPI
Program
Sanitat Animal
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