Dry formulations of the biocontrol agent Candida sakeCPA‐1 using fluidised bed drying to control the main postharvest diseases on fruits

Abstract

BACKGROUND: The biocontrol agentCandida sakeCPA-1 is effective against several diseases. Consequently, the optimisationof a dry formulation ofC. saketo improve its shelf life and manipulability is essential for increasing its potential with respectto future commercial applications. The present study aimed to optimise the conditions for making a dry formulation ofC. sakeusing a fluidised bed drying system and then to determine the shelf life of the optimised formulation and its efficacy againstPenicillium expansumon apples.RESULTS: The optimal conditions for the drying process were found to be 40∘C for 45 min and the use of potato starch as thecarrier significantly enhanced the viability. However, none of the protective compounds tested increased the viability of thedried cells. A temperature of 25∘C for 10 min in phosphate buffer was considered as the optimum condition to recover thedried formulations. The dried formulations should be stored at 4∘C and air-packaged; moreover, shelf life assays indicated goodresults after 12 months of storage. The formulated products maintained their biocontrol efficacy.CONCLUSION: A fluidised bed drying system is a suitable process for dehydratingC. sakecells; moreover, theC. sakeformulationis easy to pack, store and transport, and is a cost-effective process.© 2017 Society of Chemical Industry