Characterization of porcine dendritic cell response to Streptococcus suis
Author
Lecours, Marie-Pier
Segura, Mariela
Lachance, Claude
Mussa, Tufaria
Surprenant, Charles
Gottschalk, Marcelo
Publication date
2011-06-02ISSN
0928-4249
Abstract
Streptococcus suis is a major swine pathogen and important zoonotic agent causing mainly septicemia and
meningitis. However, the mechanisms involved in host innate and adaptive immune responses toward S. suis as well
as the mechanisms used by S. suis to subvert these responses are unknown. Here, and for the first time, the ability of
S. suis to interact with bone marrow-derived swine dendritic cells (DCs) was evaluated. In addition, the role of S. suis
capsular polysaccharide in modulation of DC functions was also assessed. Well encapsulated S. suis was relatively
resistant to phagocytosis, but it increased the relative expression of Toll-like receptors 2 and 6 and triggered the
release of several cytokines by DCs, including IL-1b, IL-6, IL-8, IL-12p40 and TNF-a. The capsular polysaccharide was
shown to interfere with DC phagocytosis; however, once internalized, S. suis was readily destroyed by DCs
independently of the presence of the capsular polysaccharide. Cell wall components were mainly responsible for DC
activation, since the capsular polysaccharide-negative mutant induced higher cytokine levels than the wild-type
strain. The capsular polysaccharide also interfered with the expression of the co-stimulatory molecules CD80/86 and
MHC-II on DCs. To conclude, our results show for the first time that S. suis interacts with swine origin DCs and
suggest that these cells might play a role in the development of host innate and adaptive immunity during an
infection with S. suis serotype 2.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
12
Publisher
BMC
Is part of
Veterinary Research
Citation
Lecours, Marie-Pier, Mariela Segura, Claude Lachance, Tufaria Mussa, Charles Surprenant, Maria Montoya, and Marcelo Gottschalk. 2011. “Characterization of Porcine Dendritic Cell Response to Streptococcus Suis.” Veterinary Research 42 (1): 72. doi:10.1186/1297-9716-42-72
Program
Sanitat Animal
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- ARTICLES CIENTÍFICS [2624]
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