Development and bin mapping of a Rosaceae Conserved Ortholog Set (COS) of markers
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Fecha de publicación
2009-11-29ISSN
1471-2164
Resumen
Background: Detailed comparative genome analyses within the economically important Rosaceae
family have not been conducted. This is largely due to the lack of conserved gene-based molecular
markers that are transferable among the important crop genera within the family [e.g. Malus
(apple), Fragaria (strawberry), and Prunus (peach, cherry, apricot and almond)]. The lack of
molecular markers and comparative whole genome sequence analysis for this family severely
hampers crop improvement efforts as well as QTL confirmation and validation studies.
Results: We identified a set of 3,818 rosaceaous unigenes comprised of two or more ESTs that
correspond to single copy Arabidopsis genes. From this Rosaceae Conserved Orthologous Set
(RosCOS), 1039 were selected from which 857 were used for the development of intron-flanking
primers and allele amplification. This led to successful amplification and subsequent mapping of 613
RosCOS onto the Prunus TxE reference map resulting in a genome-wide coverage of 0.67 to 1.06
gene-based markers per cM per linkage group. Furthermore, the RosCOS primers showed
amplification success rates from 23 to 100% across the family indicating that a substantial part of
the RosCOS primers can be directly employed in other less studied rosaceaous crops.
Comparisons of the genetic map positions of the RosCOS with the physical locations of the
orthologs in the Populus trichocarpa genome identified regions of colinearity between the genomes
of Prunus-Rosaceae and Populus-Salicaceae.
Conclusion: Conserved orthologous genes are extremely useful for the analysis of genome
evolution among closely and distantly related species. The results presented in this study
demonstrate the considerable potential of the mapped Prunus RosCOS for genome-wide marker
employment and comparative whole genome studies within the Rosaceae family. Moreover, these
markers will also function as useful anchor points for the genome sequencing efforts currently
ongoing in this family as well as for comparative QTL analyses.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
575 - Genética general. Citogenética general. Inmunogenética. Evolución. Filogenia
633 - Cultivos y producciones
Páginas
12
Publicado por
BMC
Publicado en
BMC Genomics
Citación
Cabrera, Antonio, Alex Kozik, Werner Howad, Pere Arus, Amy F Iezzoni, and Esther Van Der Knaap. 2009. “Development and Bin Mapping of a Rosaceae Conserved Ortholog Set (COS) of Markers.” BMC Genomics 10 (1): 562. doi: 10.1186/1471-2164-10-562
Program
Genòmica i Biotecnologia
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