Browsing PUBLICACIONS CIENTÍFIQUES by Author "Darji, Ayub"
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Conserved HA-peptide NG34 formulated in pCMV-CTLA4-Ig reduces viral shedding in pigs after a heterosubtypic influenza virus SwH3N2 challenge
Sisteré-Oró, Marta; Vergara-Alert, Júlia; Stratmann, Thomas; López-Serrano, Sergi; Pina-Pedrero, Sonia; Córdoba, Lorena; Pérez-Maillo, Mónica; Pleguezuelos, Patrícia; Vidal, Enric; Veljkovic, Veljko; Segalés, Joaquim; Nielsen, Jens; Fomsgaard, Anders; Darji, Ayub (PLoS ONE, 2019-03-01) Sanitat AnimalSwine influenza viruses (SIVs), the causal agents of swine influenza, are not only important to control due to the economic losses in the swine industry, but also can be pandemic pathogens. Vaccination ... -
Conserved HA-peptides expressed along with flagellin in Trichoplusia ni larvae protects chicken against intranasal H7N1 HPAIV challenge
Sisteré-Oró, Marta; Martínez-Pulgarín, Susana; Solanes, David; Veljkovic, Veljko; López-Serrano, Sergi; Córdoba, Lorena; Cordón, Ivan; Escribano, José M.; Darji, Ayub (Vaccine, 2019-11-14) Sanitat AnimalThe immunization of poultry where H5 and H7 influenza viruses (IVs) are endemic is one of the strategies to prevent unexpected zoonoses. Our group has been focused on conserved HA-epitopes as potential ... -
Conserved Synthetic Peptides from the Hemagglutinin of Influenza Viruses Induce Broad Humoral and T-Cell Responses in a Pig Model
Vergara-Alert, Julia; Argilaguet, Jordi M.; Busquets, Nuria; Ballester, Maria; Martın-Valls, Gerard E.; Rivas, Raquel; Lopez-Soria, Sergio; Solanes, David; Majo, Natalia; Segales, Joaquim; Veljkovic, Veljko; Rodrıguez, Fernando; Darji, Ayub (PLoS ONE, 2012-07-16) Sanitat AnimalOutbreaks involving either H5N1 or H1N1 influenza viruses (IV) have recently become an increasing threat to cause potential pandemics. Pigs have an important role in this aspect. As reflected in the ... -
Development of a Fully Protective Pandemic Avian Influenza Subunit Vaccine in Insect Pupae
Falcón, Ana; Martínez-Pulgarín, Susana; López-Serrano, Sergi; Reytor, Edel; Cid, Miguel; Núñez, Maria del Carmen; Córdoba, Lorena; Darji, Ayub; Escribano, José M. (Viruses, 2024-05-23) Sanitat AnimalIn this study, we pioneered an alternative technology for manufacturing subunit influenza hemagglutinin (HA)-based vaccines. This innovative method involves harnessing the pupae of the Lepidoptera ... -
Distribution patterns of influenza virus receptors and viral attachment patterns in the respiratory and intestinal tracts of seven avian species
Costa, Taiana; Chaves, Aida J; Valle, Rosa; Darji, Ayub; van Riel, Debby; Kuiken, Thijs; Majó, Natàlia; Ramis, Antonio (Veterinary Research, 2012-04-10) Sanitat AnimalThis study assessed the presence of sialic acid α-2,3 and α-2,6 linked glycan receptors in seven avian species. The respiratory and intestinal tracts of the chicken, common quail, red-legged partridge, ... -
DNA vaccine based on conserved HA-peptides induces strong immune response and rapidly clears influenza virus infection from vaccinated pigs
Sisteré-Oró, Marta; López-Serrano, Sergi; Veljkovic, Veljko; Pina-Pedrero, Sonia; Vergara-Alert, Júlia; Córdoba, Lorena; Pérez-Maillo, Mónica; Pleguezuelos, Patrícia; Vidal, Enric; Segalés, Joaquim; Nielsen, Jens; Fomsgaard, Anders; Darji, Ayub (PLoS ONE, 2019-09-25) Sanitat AnimalSwine influenza virus (SIVs) infections cause a significant economic impact to the pork industry. Moreover, pigs may act as mixing vessel favoring genome reassortment of diverse influenza viruses. ... -
Evolution of Swine Influenza Virus H3N2 in Vaccinated and Nonvaccinated Pigs after Previous Natural H1N1 Infection
López-Valiñas, Álvaro; Baioni, Laura; Córdoba, Lorena; Darji, Ayub; Chiapponi, Chiara; Segalés, Joaquim; Ganges, Llilianne; Núñez, J Ignacio (Viruses, 2022-09-10) Sanitat AnimalSwine influenza viruses (SIV) produce a highly contagious and worldwide distributed disease that can cause important economic losses to the pig industry. Currently, this virus is endemic in farms and, ... -
Exploring the Antiviral Potential of Natural Compounds against Influenza: A Combined Computational and Experimental Approach
Perovic, Vladimir; Stevanovic, Kristina; Bukreyeva, Natalya; Paessler, Slobodan; Maruyama, Junki; López-Serrano, Sergi; Darji, Ayub; Sencanski, Milan; Radosevic, Draginja; Berardozzi, Simone; Botta, Bruno; Mori, Mattia; Glisic, Sanja (International Journal of Molecular Sciences, 2024-04-30) Sanitat AnimalThe influenza A virus nonstructural protein 1 (NS1), which is crucial for viral replication and immune evasion, has been identified as a significant drug target with substantial potential to contribute ... -
Exposure to a Low Pathogenic A/H7N2 Virus in Chickens Protects against Highly Pathogenic A/H7N1 Virus but Not against Subsequent Infection with A/H5N1
Vergara-Alert, Júlia; Moreno, Ana; Zabala, Juliana G.; Bertran, Kateri; Costa, Taiana P.; Cordón, Iván; Rivas, Raquel; Majó, Natàlia; Busquets, Núria; Cordioli, Paolo; Rodriguez, Fernando; Darji, Ayub (PLoS ONE, 2013-03-04) Sanitat AnimalRecent evidences have demonstrated that the presence of low pathogenic avian influenza viruses (LPAIV) may play an important role in host ecology and transmission of avian influenza viruses (AIV). While ... -
Genetic diversification patterns in swine influenza A virus (H1N2) in vaccinated and nonvaccinated animals
López Valiñas, Álvaro; Valle, Marta; Pérez, Marta; Darji, Ayub; Chiapponi, Chiara; Ganges, Llilianne; Segalés, Joaquim; Núñez, José I. (Frontiers in Cellular and Infection Microbiology, 2023-09-15) Sanitat AnimalInfluenza A viruses (IAVs) are characterized by having a segmented genome, low proofreading polymerases, and a wide host range. Consequently, IAVs are constantly evolving in nature causing a threat ... -
Identification and Characterization of Swine Influenza Virus H1N1 Variants Generated in Vaccinated and Nonvaccinated, Challenged Pigs
López-Valiñas, Álvaro; Sisteré-Oró, Marta; López-Serrano, Sergi; Baioni, Laura; Darji, Ayub; Chiapponi, Chiara; Segalés, Joaquim; Ganges, Llilianne; Núñez, José I. (Viruses, 2021-10-16) Sanitat AnimalInfluenza viruses represent a continuous threat to both animal and human health. The 2009 H1N1 A influenza pandemic highlighted the importance of a swine host in the adaptation of influenza viruses to ... -
Immune Responses to Pandemic H1N1 Influenza Virus Infection in Pigs Vaccinated with a Conserved Hemagglutinin HA1 Peptide Adjuvanted with CAF®01 or CDA/αGalCerMPEG
López-Serrano, Sergi; Cordoba, Lorena; Pérez-Maillo, Mónica; Pleguezuelos, Patricia; Remarque, Edmond J.; Ebensen, Thomas; Guzmán, Carlos A.; Christensen, Dennis; Segalés, Joaquim; Darji, Ayub (Vaccines, 2021-07-06) Sanitat AnimalThis study aimed to evaluate the immune response and protection correlates against influenza virus (IV) infection in pigs vaccinated with the novel NG34 HA1 vaccine candidate adjuvanted with either ... -
Influenza NG-34 T cell conserved epitope adjuvanted with CAF01 as a possible influenza vaccine candidate
Sisteré-Oró, Marta; Pedersen, Gabriel K.; Córdoba, Lorena; López-Serrano, Sergi; Christensen, Dennis; Darji, Ayub (Veterinary Research, 2020-04-20) Sanitat AnimalConserved epitopes are targets commonly researched to be part of universal vaccine candidates against influenza viruses (IV). These conserved epitopes need to be cross-protecting against distinct IV ... -
Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens
Chaves, Aida J.; Busquets, Núria; Valle, Rosa; Rivas, Raquel; Vergara-Alert, Júlia; Dolz, Roser; Ramis, Antonio; Darji, Ayub; Majó, Natàlia (Veterinary Research, 2011-10-07) Sanitat AnimalIn order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into the central nervous system (CNS) of chickens, specific pathogen free chickens were ... -
Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
Bertran, Kateri; Pérez-Ramírez, Elisa; Busquets, Núria; Dolz, Roser; Ramis, Antonio; Darji, Ayub; Abad, Francesc Xavier; Valle, Rosa; Chaves, Aida; Vergara-Alert, Júlia; Barral, Marta; Höfle, Ursula; Majó, Natàlia (Veterinary Research, 2011-02-07) Sanitat AnimalAn experimental infection with highly pathogenic avian influenza virus (HPAIV) and low pathogenic avian influenza virus (LPAIV) was carried out in red-legged partridges (Alectoris rufa) in order to ... -
Protective effect of a polyvalent influenza DNA vaccine in pigs
Karlsson, Ingrid; Borggren, Marie; Rosenstierne, Maiken Worsøe; Trebbien, Ramona; Williams, James A.; Vidal, Enric; Vergara-Alert, Júlia; Solanes Foz, David; Darji, Ayub; Sisteré-Oró, Marta; Segalés, Joaquim; Nielsen, Jens; Fomsgaard, Anders (Veterinary Immunology and Immunopathology, 2017-11-23) Sanitat AnimalBackground: Influenza A virus in swine herds represents a major problem for the swine industry and poses a constant threat for the emergence of novel pandemic viruses and the development of more effective ... -
Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus
Mohamed, Walid; Sethi, Shneh; Tchatalbachev, Svetlin; Darji, Ayub; Chakraborty, Trinad (PLoS ONE, 2012-04-19) Sanitat AnimalIn this study we propose a novel bacterial vaccine strategy where non-pathogenic bacteria are complemented with traits desirable for the induction of protective immunity. To illustrate the proof of ... -
Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring
López-Serrano, Sergi; Neila-Ibáñez, Carlos; Costa-Hurtado, Mar; Mahmmod, Yasser; Martínez-Martínez, Jorge; Galindo-Cardiel, Iván José; Darji, Ayub; Rodríguez, Fernando; Sibila, Marina; Aragon, Virginia (Vaccines, 2021-05-20) Sanitat AnimalGlaesserella (Haemophilus) parasuis, an early colonizer of the nasal cavity in piglets, is a highly heterogeneous species, comprising both commensal and virulent strains. Virulent G. parasuis strains ... -
Vaccination against swine influenza in pigs causes different drift evolutionary patterns upon swine influenza virus experimental infection and reduces the likelihood of genomic reassortments
López Valiñas, Álvaro; Valle, Marta; Wang, Miaomiao; Darji, Ayub; Cantero, Guillermo; Chiapponi, Chiara; Segalés, Joaquim; Ganges, Llilianne; Núñez, J Ignacio (Frontiers in Cellular and Infection Microbiology, 2023-03-13) Sanitat AnimalInfluenza A viruses (IAVs) can infect a wide variety of bird and mammal species. Their genome is characterized by 8 RNA single stranded segments. The low proofreading activity of their polymerases and ... -
VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages
Costa-Hurtado, Mar; Ballester, Maria; Galofré-Milà, Nuria; Darji, Ayub; Aragon, Virginia (Veterinary Research, 2012-07-27) Sanitat AnimalHaemophilus parasuis, a member of the family Pasteurellaceae, is a common inhabitant of the upper respiratory tract of healthy pigs and the etiological agent of Glässer’s disease. As other virulent ...