A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts
Ver/Abrir
Autor/a
Olsvik, Pål A.
Roderick, Nigel Finn
Remø, Sofie C.
Fjelldal, Per G.
Chauvigné, François
Glover, Kevin A.
Hansen, Tom
Waagbø, Rune
Fecha de publicación
2020-07-28ISSN
0014-4835
Resumen
To avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology compared to diploid fish, with increased prevalence of vertebral deformities and ocular cataracts as two of the most severe disorders. Here, we investigated the mechanisms behind the higher prevalence of cataracts in triploid salmon, by comparing the transcriptional patterns in lenses of diploid and triploid Atlantic salmon, with and without cataracts. We assembled and characterized the Atlantic salmon lens transcriptome and used RNA-seq to search for the molecular basis for cataract development in triploid fish. Transcriptional screening showed only modest differences in lens mRNA levels in diploid and triploid fish, with few uniquely expressed genes. In total, there were 165 differentially expressed genes (DEGs) between the cataractous diploid and triploid lens. Of these, most were expressed at lower levels in triploid fish. Differential expression was observed for genes encoding proteins with known function in the retina (phototransduction) and proteins associated with repair and compensation mechanisms. The results suggest a higher susceptibility to oxidative stress in triploid lenses, and that mechanisms connected to the ability to handle damaged proteins are differentially affected in cataractous lenses from diploid and triploid salmon.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
639 - Caza. Pesca. Piscicultura
Páginas
14
Publicado por
Elsevier
Publicado en
Experimental Eye Research
Citación
Olsvik, Pål A., Roderick Nigel Finn, Sofie C. Remø, Per G. Fjelldal, François Chauvigné, Kevin A. Glover, Tom Hansen, and Rune Waagbø. 2020. "A Transcriptomic Analysis Of Diploid And Triploid Atlantic Salmon Lenses With And Without Cataracts". Experimental Eye Research 199: 108150. doi:10.1016/j.exer.2020.108150.
Program
Aqüicultura
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2555]
El ítem tiene asociados los siguientes ficheros de licencia:
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by/4.0/