Deciphering the Monilinia fructicola Genome to Discover Effector Genes Possibly Involved in Virulence
Visualitza/Obre
Autor/a
Vilanova, Laura
Valero-Jiménez, Claudio A.
van Kan, Jan A.L.
Data de publicació
2021-04-14ISSN
2073-4425
Resum
Brown rot is the most economically important fungal disease of stone fruits and is primarily caused by Monilinia laxa and Monlinia fructicola. Both species co-occur in European orchards although M. fructicola is considered to cause the most severe yield losses in stone fruit. This study aimed to generate a high-quality genome of M. fructicola and to exploit it to identify genes that may contribute to pathogen virulence. PacBio sequencing technology was used to assemble the genome of M. fructicola. Manual structural curation of gene models, supported by RNA-Seq, and functional annotation of the proteome yielded 10,086 trustworthy gene models. The genome was examined for the presence of genes that encode secreted proteins and more specifically effector proteins. A set of 134 putative effectors was defined. Several effector genes were cloned into Agrobacterium tumefaciens for transient expression in Nicotiana benthamiana plants, and some of them triggered necrotic lesions. Studying effectors and their biological properties will help to better understand the interaction between M. fructicola and its stone fruit host plants.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
633 - Cultius i produccions
Pàgines
15
Publicat per
MDPI
Publicat a
Genes
Citació
Vilanova, Laura, Claudio A. Valero-Jiménez, and Jan A.L. van Kan. 2021. "Deciphering The Monilinia Fructicola Genome To Discover Effector Genes Possibly Involved In Virulence". Genes 12 (4): 568. doi:10.3390/genes12040568.
Número de l'acord de la subvenció
EC/H2020/741964/EU/The role of necrotrophic effectors in the ability of Botrytis and Monilinia species to infect host plants/NECROFUNGI
Programa
Postcollita
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